Web7 Dec 2024 · Briefly, the DNA was sheared using the Megaruptor2 kit to generate 15 to 20-Kbp (kilobase pair) fragments. The BluePippin SMRTbell Library Kit was then used to select DNA fragments longer than 15 Kbp, which were used as input to the SMRTbell Express Template Prep Kit 2.0. The resulting PacBio HiFi SMRTbell libraries were sequenced … Web26 May 2024 · 136 SMRTbell Express Template Prep Kit 2.0: Procedure & Checklist, PN 101-853-100, version 03. The 137 only exception to this protocol was that the gDNA was not sheared using the Megaruptor 3 system, 138 but rather using the Megaruptor 2 system (Diagenode) with the software settings at 20Kb and 139. and ...
High Molecular Weight DNA Extraction NEB
Web10x Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1, Visium Spatial Gene Expression libraries, and PacBio Iso-Seq SMRTbell Express Template Prep Kit 2.0 Simple 3-step protocol integrated into 10x Genomics® Chromium™ Next GEM Single Cell 3’ workflow nioh 2 graphics settings
Technical Overview: Full-Length 16S Library Preparation Using SMRTbell …
Web4 Mar 2024 · · PACBIO® SMRTbell® Express Template Prep Kit 2.0 (Part No. 100-938-900) Suggested items for rapid processing ... Use the Express Template Prep Kit 2.0 for sequencing Shoreline Biome V1-V9 or StrainID™ on the Sequel and Sequel II Systems. Users should refer to the “Procedure & Checklist – Amplification of Full-Length 16S Gene with ... Web6 Jan 2024 · selected DNA was used to prepare a SMRTbell library using the SMRTbell Express Template Prep Kit 2.0 as recommended by the manufacturer (Pacific Biosciences, Menlo Park, CA, USA). The library was sequenced in two SMRT Cell 8M cells on a Sequel II instrument using Sequel Sequencing Kit 3.0, Web30 Sep 2024 · for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The resultant library was separated on BluePippin (Sage Science) to remove short DNA fragments (<20 kb), and sequenced with SMRT cell 8 M on Sequel II and Sequel IIe systems (PacBio). number one china lopatcong nj