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Phis2自激活

WebbpHIS2-p53+pGADT7-53m（阳性对照） pHIS2+pGADT7-53m（阴性对照） 服务特色：经验足 周期短 性价比高 通量高. 客户提供. 构建酵母文库材料. 细胞样本：细胞数量＞ 1*10^7 动物样本：＞lg . 植物样本：＞ 2g 总RNA: ＞200μg. 酵母单杂筛库材料. 启动子序列 含有诱饵 … http://html.rhhz.net/SWJSTB/html/2024-12-110.htm

常用测序通用引物大全 纽普生物

Webb22 juli 2013 · Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. It contains a HIS3 nutritional reporter gene, located downstream of a … Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. culpeper county government jobs https://segnicreativi.com

酵母表达载体构件 – 聚生物

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb24 apr. 2016 · Figure 酵母单杂和双杂筛选的一般步骤，更加详细单杂和双杂筛选流程图，请参考Figures BDMatchmaker TM 单杂和双杂文库筛选与构建. 如这个图表所示，As diagramshows,重组介导克隆使文库构建和筛选更快捷高效， 尽管这里没有显示，双杂文库能通过酵母融合筛选（细节 ... Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). east haven ct lexus

酵母双杂交 AH109,Y187_ATCC菌株-上海北诺生物科技有限公司

Category:pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

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Phis2自激活

p53-His2 plasmid,p53-His2,p53-His2 plasmid,p53-His2 sequence,p53-His2 …

Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a multiple ... Webb酵母是单细胞低等的真核生物，具有生长快、成本低、易于遗传操作，能对外源蛋白进行翻译后加工和修饰、不产生有毒产物等特点，认为是外源蛋白的合适宿主 [1] 。 酵母分为三类：第一是酿酒酵母；第二是栗酒裂殖酵母；第三是非常规酵母，主要有巴氏毕赤酵母、乳酸克鲁维亚酵母等 [2] 。

Phis2自激活

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http://www.biogenesci.com/ Webb26 maj 2024 · pHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication sequence (ARS4) and TRP1 nutritional marker for replication …

Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … Webb5 nov. 2015 · 酵母双杂交 AH109,Y187. 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供，载体来源于 promega,addgene,clontech,novagen,invitrogen 可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株, 并可提供实验技术外包服务, 如基因克隆、质粒构建、蛋白原核 ...

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Webb先下结论，不存在可以预测蛋白是否互作的预测方法，小分子和蛋白互作可以通过计算MM-PBSA结合自由能来预测，蛋白-蛋白是否互作需要借助实验手段如IP、等温滴定量热（ITC）或者表面等离子共振（SPR）。. 有了实验基础，可以通过模拟的方式预测蛋白的结 …

Webb泰斯拓生物向社会各界提供古菌、细菌、放线菌、酵母菌、丝状真菌等共享菌株资源，. 包括模式菌株、标准菌株（质控菌株）、生产菌株、以及用于研发的菌株，. 覆盖了科研、教育、农林、工业、海洋等行业的科研、生产和检验用菌株。. 且我司提供国外各 ... culpeper county health department culpeper vaWebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … east haven ct online assessor databaseWebb1 mars 2024 · 测序通用引物列表. 引物名称. 序列 (5'→3') 描述. 3'AOX1. GCAAATGGCATTCTGACATCC. For Pichia vectors with AOX1 terminator, reverse primer. 5'AOX1. GACTGGTTCCAATTGACAAGC. east haven ct patchWebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 east haven ct mayor\u0027s officeWebb服务时间：24小时在线客服 . 客服热线：400-000-7085 culpeper county government officesWebb12 apr. 2024 · 方法：以启动子a构建到酵母单杂交启动子载体phis2，文库质粒为pgadt7-中山杉文库。 材料： 1．启动子克隆：pHIS2-A。 根据实验需求进行扩增并酶切构建载 … east haven ct property appraiserWebb23 apr. 2024 · 答：标准设置为30℃孵育30 min，然后42℃热激20 min。 孵育时间在3 h以内，热激不超过30 min都可以得到不错的转化效果。 Coolaber的Super转化Kit经过优化及检测证明，将孵育热激合并，条件设定为30℃，45-60 min同样能达到理想的转化效果。 问6：转化产物用无菌水和生理盐水重悬有什么区别？ 答：重悬后直接涂板，生理盐水和无菌水 … culpeper county internet providers