Phee401e-mcherry

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August undefined, 2024

WebAug 16, 2024 · Building on the pHEE401E plasmid, a Cambia T-DNA adapted for 96 genome editing with CRISPR/Cas9 nucleases (Wang & al., 2015), we created a series of 97 vectors … WebThe pHEE401E_UBQ_Bar vector is a version in which the egg-speciﬁc promoter for Cas9 expression in the pHEE401E vector was replaced with the UBQ10 promoter, and the hygromycin resistance gene was ...

Selective inheritance of target genes from only one parent of …

WebDownload scientific diagram Validation of the CRISPR/Cas toolkit in Arabidopsis . (A) Physical maps of the T-DNAs of two pGreen-derived CRISPR/Cas9 binary vectors, each carrying two-gRNAs ... WebJan 1, 2024 · The mCherry-based method provides an efficient and reliable approach to quickly identify transgene-free plants whose genomes have been properly edited. … tara fowler montel williams

mCherry :: Fluorescent Protein Database

WebDec 12, 2024 · The pHEE401E-mCherry vector was generated by fusing the seed-specific At2S3 promoter–driven mCherry to the pHEE401E vector skeleton (Xing et al. 2014, Wang … WebMay 1, 2024 · For fC-0029, the fCas9 fragment was retrieved as Spe I/EcoR I fragments from PHEE401E and inserted into pGreen0029. To construct pGHN-R, the reference gene … WebNov 20, 2024 · The CRISPR genome-editing system includes two key components: an sgRNA that specifically recognizes the target DNA, and a Cas9 endonuclease that precisely … tara fowler williams

Targeted mutagenesis of the Arabidopsis GROWTH

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pHEE401E (Plasmid #71287) Print Enlarge View all sequences Purpose Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance Depositing Lab Qi-Jun Chen Publication Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. tara frye slatington paWebJan 6, 2024 · Both the first and second constructs were cloned in the pHEE401E vector, which contains a maize codon-optimized Cas9 gene (zCas9) driven by an Arabidopsis egg-cell specific promoter (E.C.1.1) fused with an egg-cell specific enhancer (E.C.1.2) [ 21 ]. Fig. 1 Schematic illustration of gRNA target sites and the two CRISPR multiplexing constructs. a. tara fry photography

"WebApr 10, 2024 · The construction of pHEE401E-CAP1 was described and unpublished work in our lab. Positive transgenic lines were screened on solid Murashige and Skoog (MS) medium containing 20 μg/mL hygromycin and confirmed by sequencing. ... A co-expression assay showed that GFP-CAP1 co-localized with VDAC3-mCherry, a mitochondrial marker (Fig. 5 … " - Phee401e-mcherry

Phee401e-mcherry

WebAug 16, 2024 · Building on the pHEE401E plasmid, a Cambia T-DNA adapted for 96 genome editing with CRISPR/Cas9 nucleases (Wang & al., 2015), we created a series of 97 vectors enabling selection and counter-selection of transgenics on the basis of seed 98 fluorescence. Toward this end, we replaced the hygromycin resistance marker of pHEE401E Web结果表明，本实验成功构建了编辑载体pHEE401E- mCherry - CKX3，拟南芥转化后代中目标基因成功被高效编辑， CKX3 的纯合突变植株表型与野生型差异明显，内源激素含量差异 …

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http://www.biofeng.com/adjy/zhili/pHEE401E.html WebmCherry Antibody (M11217) in ICC/IF. Immunofluorescent analysis of mCherry Tag was performed using 70% confluent mCherry-H3 transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature.

WebAug 17, 2024 · The transgenic lines were screened by B asta for pB7WG2.0 and pCB302, and by hygromycin for pHEE401E. ... Fluorescence was excited at 488 nm for Venus, and 556 nm for mCherry, and captured at 520–550 nm for Venus and 590–630 nm for mCherry. To harvest intercellular fluid, three leaves of N. benthamiana plants were infiltrated with 100 … WebAn mCherry gene under the control of a seed-specific At2S3 promoter was cloned into the plasmid pHEE401E to generate pHEE401E-mCherry14,16. The two gRNA units, which …

WebZepole is your one-stop partner for all of your foodservice needs! From custom kitchen design with equipment delivery and installation; to every day needs like dinnerware, … Web1. College of Life and Geographic Sciences，The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region，Kashi University，Kashi 844000 2. College of Biological and Food Engineering，Guangdong University of Petrochemical …

WebDec 7, 2024 · 56 usually between 30% and 40% of successful protein expression in E. coli [18]. However, when mCherry 57 is used as a reporter, we observe fluorescence in about 65% of E. coli clones (Supplemental Figure S1). 58 Additionally, a large-scale analysis of the transcription start sites (TSS) of these clones showed a non-

WebFor this purpose we cloned mCherry ( BBa_J06504) into the purification and expression vector pTXB1 from NEB and at the same time added six histidines to the C-terminus of mCherry in pSB1C3 ( BBa_K2926048 ). Both expression vectors were transformed in … tara freeman chattanoogaWebJun 22, 2024 · The GFP gene is designed to fuse with part of CRY1 in frame, providing a visual marker for precise insertion of the gene drive element. The mCherry is another … tara fry inspired teacherWebHave a question, comment, or need assistance? Send us a message or call (630) 833-0300. Will call available at our Chicago location Mon-Fri 7:00am–6:00pm and Sat … tara furman knowing god ministries