Flag antibody proteintech
WebApr 8, 2024 · The antibodies used in IHC analysis are listed in Supplementary Table S4. ... E. Coli derived GST-Vector or GST-PRDX1 fusion protein was purified and prepared by Proteintech (Wuhan, China). Briefly, the total protein of 293T cells with or without Flag-tagged IRAK1 overexpression was incubated with GST-PRDX1 or GST-Vector fusion … WebThe primary antibodies against α-actinin (# 11313-2-AP) and Flag (DYKDDDDK) tag (# 20543-1-AP) were bought in Proteintech Group (Chicago, IL, USA). CCL4 (# ab45690) and ADAMTS5 (# ab41037) were obtained. (12) SIRT2 promotes the viability, invasion and metastasis of osteosarcoma cells by inhibiting the degradation of Snail Cell Death Dis ...
Flag antibody proteintech
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WebApr 6, 2024 · Long-term memory formation requires de novo RNA and protein synthesis. By using the differential display-polymerase chain reaction strategy, we have presently identified the Nedd4 family interacting protein 1 (Ndfip1) cDNA fragment that is differentially expressed between the slow learners and the fast learners from the water maze learning … WebThe proteins retained on beads were subjected to immunoblot using anti-GST and anti-Flag antibodies. F 293T cells were transfected with si-Control or si-p62 under MG132 treatment. ... (1:5000; SA00001-1), and goat polyclonal anti-rabbit IgG (1:5000; SA00001-2) were obtained from Proteintech; antibody against pan–acetyl-lysine (1:2000, 9441S ...
WebTag (His-, HA-, Flag-. GFP) fusion 단백질 검출 시 당사에서 일차항체 제공; 특이 일차항체는 고객이 제공원칙 (당사 보유 중인 특이 일차항체는 제공가능: 추가 charge 있음) 모든 이차항체는 당사 보유 항체로 진행가능; 기본분석 샘플 수: 7 samples / one blot WebMay 25, 2016 · For immunofluorescence staining, the rabbit polyclonal antibody against EGFP (1:1000, ab99955, abcam) and monoclonal anti-flag ® M2 antibody produced in mouse (1:1000, F1804, Sigma, Ronkonkoma, NY, USA) were used to probe A1CF overnight at 4 °C, At the meantime, we detected rabbit IgG by the goat anti rabbit IgG-CFL 488 …
WebFLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been … WebThese antibodies have been verified by Relative expression and Cell treatment to confirm specificity to DYKDDDDK Tag. Find the DYKDDDDK Tag antibody that fits your needs. …
WebProteintech mouse anti flag dykddddk antibody Mouse Anti Flag Dykddddk Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebThe 3xFLAG ® system is an improvement upon the original system by fusing three tandem FLAG ® epitopes for a total of 22 amino acids (Figure 1).Detection of fusion proteins containing 3xFLAG ® is enhanced up to 200 times more than any other system. Like the original FLAG tag, 3xFLAG ® is hydrophilic, contains an Enterokinase cleavage site, and … cshop uchicago winter hoursWebProteintech Anti-DDDDK Tag Polyclonal, Catalog # 20543-1-AP. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. ... adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are ... eagle arms gun show oaks pennsylvaniaWebGeneral description. The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide … eagle arms gun shopWebApr 13, 2024 · (A) HEK293T cells were infected with PR8 virus at a multiplicity of infection (MOI) of 0.01 or transfected with Flag-NS1 and vector for 24 hours, cell lysates were immunoprecipitated with an anti-Flag or anti-NS1 antibody and then analyzed by silver stain. (B-C) HEK293T cells were transfected with the indicated plasmids for 24 h. csh or bashWebMouse Anti Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … eagle arms gun shows 2020WebFor in vitro co-immunoprecipitation (co-IP) analysis, FLAG-tagged proteins were produced in HTR-8/Svneo and JAR cells transfected with LV-PADI6. Total cell lysates were incubated overnight at 4°C with anti-YAP1 antibodies (Proteintech, Wuhan, China), anti-FLAG antibodies (Sigma, MO, USA), or normal IgG (Beyotime, Shanghai, China) as a control. eagle arms lower receiverWebAug 3, 2024 · The signals were detected with anti-GFP and -FLAG antibodies (Proteintech). Cell-free degradation assay. Two-week-old seedlings of wild type (DJ) or sapk9 mutant were used to extract protein. Total protein was extracted in the degradation buffer (25 mM Tris-HCl, pH 7.5, 10 mM NaCl, 10 mM MgCl 2, 5 mM DTT, and 10 mM … eagle arms m15a2 for sale